1UYK
Human Hsp90-alpha with 8-Benzo[1,3]dioxol-,5-ylmethyl-9-butyl-2-fluoro-9H-purin-6-ylamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.840, 90.663, 98.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.420 - 2.200 |
Rwork | 0.183 |
R-free | 0.21814 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yer |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.073 | 0.291 |
Number of reflections | 9549 | |
<I/σ(I)> | 7 | 2 |
Completeness [%] | 95.9 | 88.4 |
Redundancy | 1.39 | 1.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 25% PEG MME 2000, 0.1M NA CACODYLATE, PH6.5, 0.2M MGCL2., pH 6.50 |