1UXM
A4V mutant of human SOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.374, 145.582, 112.497 |
| Unit cell angles | 90.00, 120.05, 90.00 |
Refinement procedure
| Resolution | 27.000 - 1.900 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.24900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hl5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.780 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 * | 0.256 * |
| Number of reflections | 245475 * | |
| <I/σ(I)> | 16 | 2.2 |
| Completeness [%] | 96.7 * | 70.7 * |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 0.2 M CA ACET, 15% PEG 2000, 0.1 M TRIS PH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 15 (%) | |
| 3 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.0 |
