1UW4
The structural basis of the interaction between nonsense mediated decay factors UPF2 and UPF3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-16 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9393, 0.9792, 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.306, 100.178, 153.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.100 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.064 | 0.305 * |
| Total number of observations | 338871 * | |
| Number of reflections | 79316 | |
| <I/σ(I)> | 13.74 | 4.3 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 20 * | 100 MM AMMONIUM ACETATE, 15 MM MAGNESIUM ACETATE 50 MM SODIUM CACODYLATE 6.5, 8% ISOPROPANOL, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 6 | 1 | reservoir | magnesium acetate | 15 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
| 8 | 1 | reservoir | isopropanol | 8 (%(v/v)) |
