1UW4

The structural basis of the interaction between nonsense mediated decay factors UPF2 and UPF3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-16
Detector	ADSC CCD
Wavelength(s)	0.9393, 0.9792, 0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	70.306, 100.178, 153.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.205
Rwork	0.204
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.120 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE/RESOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.100
High resolution limit [Å]	1.950	1.950
Rmerge	0.064	0.305 *
Total number of observations	338871 *
Number of reflections	79316
<I/σ(I)>	13.74	4.3
Completeness [%]	99.5	98.6
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	20 *	100 MM AMMONIUM ACETATE, 15 MM MAGNESIUM ACETATE 50 MM SODIUM CACODYLATE 6.5, 8% ISOPROPANOL, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.0
3	1	drop		100 (mM)
4	1	drop	beta-mercaptoethanol	10 (mM)
5	1	reservoir	ammonium acetate	100 (mM)
6	1	reservoir	magnesium acetate	15 (mM)
7	1	reservoir	sodium cacodylate	50 (mM)	pH6.5
8	1	reservoir	isopropanol	8 (%(v/v))