1UVN
The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 ca2+ inhibition complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 150 |
| Collection date | 2002-04-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.504, 92.134, 140.755 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.000 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hhs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.151 | 0.557 |
| Total number of observations | 880290 * | |
| Number of reflections | 51586 | |
| <I/σ(I)> | 11.9 | 1.6 |
| Completeness [%] | 96.9 | 78.4 |
| Redundancy | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.7 * | 293 * | Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.7 |
