1UVK

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 dead-end complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	150
Collection date	2000-11-15
Spacegroup name	P 1 21 1
Unit cell lengths	106.290, 93.800, 140.770
Unit cell angles	90.00, 101.35, 90.00

Refinement procedure

Resolution	20.000 * - 2.500 *
R-factor	0.243
Rwork	0.243
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hhs
RMSD bond length	0.017
RMSD bond angle	23.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.136 *	0.185
Total number of observations	4701397 *
Number of reflections	97851 *
<I/σ(I)>	25.3	3.4
Completeness [%]	97.7	96 *
Redundancy	48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.7 *	293 *	Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	MES	0.1 (M)	pH6.7