1USY

ATP phosphoribosyl transferase (HisG:HisZ) complex from Thermotoga maritima

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	101.580, 134.403, 154.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.520
R-factor	0.207
Rwork	0.203
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.798
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.055	0.289
Number of reflections	52743
<I/σ(I)>	18.44	6.86
Completeness [%]	98.4	98.1
Redundancy	17.6	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2		pH 4.20