1USL
Structure Of Mycobacterium tuberculosis Ribose-5-Phosphate Isomerase, RpiB, Rv2465c, Complexed With Phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.420, 102.390, 69.610 |
| Unit cell angles | 90.00, 95.68, 90.00 |
Refinement procedure
| Resolution | 81.650 - 1.880 |
| R-factor | 0.17019 |
| Rwork | 0.168 |
| R-free | 0.20200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nn4 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.167 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.069 | 0.335 |
| Total number of observations | 358802 * | |
| Number of reflections | 73429 | |
| <I/σ(I)> | 9.6 | 2.5 |
| Completeness [%] | 95.6 | 90.6 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 * | 20 * | 1.26 M NAKPO4 PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium potassium phosphate | 1.26 (M) | pH7.5 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | MES | 20 (mM) | pH6. |
| 5 | 1 | drop | EDTA | 0.1 (mM) | |
| 6 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 7 | 1 | drop | glycerol | 10 (%) |
