1USH
5'-NUCLEOTIDASE FROM E. COLI
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.610, 83.610, 181.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.730 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5'-NUCLEOTIDASE FROM E. COLI (ORTHORHOMBIC CRYSTAL FORM) |
RMSD bond length | 0.014 |
RMSD bond angle | 23.890 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.84) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.035 * | 0.130 * |
Number of reflections | 68495 | |
<I/σ(I)> | 19 | |
Completeness [%] | 98.4 | 93.6 |
Redundancy | 2.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | pH 6.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | 0.05 (mM) | ||
5 | 1 | reservoir | sodium acetate | 100 (mM) | |
6 | 1 | reservoir | 300 (mM) | ||
7 | 1 | reservoir | 60 (%sat) |