1URC
Cyclin A binding groove inhibitor Ace-Arg-Lys-Leu-Phe-Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.630, 113.510, 155.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.000 - 2.600 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.25500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fin |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.520 * |
Total number of observations | 113421 * | |
Number of reflections | 46681 * | |
<I/σ(I)> | 5.8 | 1 |
Completeness [%] | 98.9 * | 99.4 |
Redundancy | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 17 * | Kontopidis, G., (2003) Structure, 11, 1537. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-8 (mg/ml) | |
2 | 1 | drop | HEPES | 40 (mM) | pH7.0 |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | PEG3350 | 18 (%) | |
6 | 1 | reservoir | trisodium citrate | 100 (mM) |