1UQU
Trehalose-6-phosphate from E. coli bound with UDP-glucose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.905, 102.310, 118.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gz5 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.490 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 | 0.449 |
Number of reflections | 69411 | |
<I/σ(I)> | 12.4 | 1.79 |
Completeness [%] | 94.0 | 68 |
Redundancy | 3.10 * | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 | 30% PEG 4000, 200MM AMMONIUM ACETATE, 100MM TRISHCL PH 8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH8. |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | protein | 10 (mg/ml) |