1UPS
GlcNAc[alpha]1-4Gal releasing endo-[beta]-galactosidase from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97930 |
Spacegroup name | P 63 |
Unit cell lengths | 160.430, 160.430, 86.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.820 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.20400 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.219 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE/RESOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.058 | 0.218 |
Number of reflections | 107796 | |
<I/σ(I)> | 25.1 | 8.1 |
Completeness [%] | 95.8 | 94.4 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 42% AMMONIUM SULFATE, 2% PEG400, 0.1M HEPES-NA AT PH 7.4 |