1UPM

ACTIVATED SPINACH RUBISCO COMPLEXED WITH 2-CARBOXYARABINITOL 2 BISPHOSPHAT AND CA2+.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	279
Detector technology	CCD
Collection date	2000-05-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	219.600, 220.942, 116.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.230
R-free	0.23000 *
Structure solution method	OTHER
RMSD bond length	0.014 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.083	0.150
Total number of observations	2722153 *
Number of reflections	226289
<I/σ(I)>	15.9	4.1
Completeness [%]	90.2 *	70.6
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		10% PEG 4000, 0.1 M HEPES PH 7.8, 10 MM CACL2, 0.2 M NACL, 50 MM NAHCO3, 1MM 2-CABP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		50 (mM)
10	1	reservoir	PEG4000	10-14 (%(w/v))
2	1	drop		10 (mM)
3	1	drop	HEPES	0.1 (M)	pH7.8
4	1	drop	protein	60 (mg/ml)
5	1	drop	3PGA	100 (mM)
6	1	reservoir	HEPES	0.1 (M)	pH7.8
7	1	reservoir		0.1 (M)
8	1	reservoir		10 (mM)
9	1	reservoir		0.1-0.2 (M)