1UOL
Crystal structure of the human p53 core domain mutant M133L/V203A/N239Y/N268D at 1.9 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.000, 71.000, 104.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.600 - 1.900 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.600 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.083 | 0.290 |
Total number of observations | 231945 * | |
Number of reflections | 38078 | |
<I/σ(I)> | 16.4 | 5.5 |
Completeness [%] | 98.7 | 96.5 |
Redundancy | 6.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.6 * | 17 * | pH 7.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.2 (mg/ml) | |
2 | 1 | drop | FL-CDB3 | 250000 (nm) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | Tris | 20 (mM) | pH7.6 |
6 | 1 | reservoir | HEPES | 100 (mM) | pH7.2 |
7 | 1 | reservoir | dithiothreitol | 10 (mM) | |
8 | 1 | reservoir | PEG4000 | 19 (%(w/v)) |