1UNQ

High resolution crystal structure of the Pleckstrin Homology Domain Of Protein Kinase B/Akt Bound To Ins(1,3,4,5)-Tetrakisphophate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	82.904, 34.388, 44.292
Unit cell angles	90.00, 115.29, 90.00

Refinement procedure

Resolution	15.000 - 0.980
R-factor	0.154
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h10
RMSD bond length	0.014
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.020
High resolution limit [Å]	0.980	0.980
Rmerge	0.064	0.557
Number of reflections	60005
<I/σ(I)>	19.3	2.8
Completeness [%]	93.2	92.9
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		0.25 M AMMONIUM ACETATE, 30 % PEG 4000, 0.1 M SODIUM ACETATE (4.6), pH 4.60