1UDT
Crystal structure of Human Phosphodiesterase 5 complexed with Sildenafil(Viagra)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.122, 155.632, 89.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.300 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.25100 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.880 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16848 | |
| Completeness [%] | 86.7 | 48.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 8000, Magnesium chloride, Tris buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
