1U9S
Crystal structure of the specificity domain of Ribonuclease P of the A-type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.470, 120.890, 133.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.900 |
| R-factor | 0.2414 |
| Rwork | 0.241 |
| R-free | 0.27450 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 13911 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | 1,6 hexandiol, barium chloride, spermine, HEPES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 1,6 hexandiol | ||
| 2 | 1 | 1 | spermine | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | barium chloride | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | spermine | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | barium chloride | ||
| 9 | 1 | 2 | H2O |
