1U8R
Crystal Structure of an IdeR-DNA Complex Reveals a Conformational Change in Activated IdeR for Base-specific Interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 31 |
| Unit cell lengths | 107.959, 107.959, 215.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.750 - 2.750 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FX7 and 1DDN |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 63753 | |
| <I/σ(I)> | 10.87 | 2.09 |
| Completeness [%] | 86.5 | 65.5 |
| Redundancy | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 7% w/v PEG 8000, 0.2 M calcium acetate, 0.1 M imidazole, 0.01 M cobalt chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | calcium acetate | ||
| 3 | 1 | 1 | imidazol | ||
| 4 | 1 | 1 | cobalt chloride | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 8000 | ||
| 7 | 1 | 2 | calcium acetate | ||
| 8 | 1 | 2 | cobalt chloride | ||
| 9 | 1 | 2 | H2O |
