1U7W
Phosphopantothenoylcysteine synthetase from E. coli, CTP-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Detector technology | CCD |
Collection date | 2003-03-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 45.926, 144.406, 246.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.225 |
Rwork | 0.211 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u7u |
RMSD bond length | 0.011 |
RMSD bond angle | 1.620 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.065 |
Number of reflections | 28789 |
Completeness [%] | 99.5 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | PEG 3000, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |