1U74
Electron Transfer Complex between cytochrome C and cytochrome C peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2003-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.810, 111.624, 87.518 |
| Unit cell angles | 90.00, 104.14, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.26129 |
| Rwork | 0.260 |
| R-free | 0.28728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.192 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 36481 | |
| <I/σ(I)> | 9.5 | 2.84 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 3350, sodium chloride, n-octyl-beta-D-glucoside, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
