1U6J
The Structure of native coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase at 2.4A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.900, 118.900, 219.200 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.175 |
R-free | 0.23500 |
Starting model (for MR) | Selenomethionine-labelled METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE (MTD) PDB-ID 1QV9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.250 |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.108 | 0.302 |
Number of reflections | 144106 | |
<I/σ(I)> | 14.2 | 5.9 |
Completeness [%] | 95.0 | 88.6 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MOPS, KOH, MPD, PEG 400, Mg acetate, Na phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |