1U6I
The Structure of native coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase at 2.2A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.900, 118.900, 219.200 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 19.350 - 2.200 |
Rwork | 0.189 |
R-free | 0.23500 |
Starting model (for MR) | Selenomethionine-labelled methylenetetrahydromethanopterin dehydrogenase (MTD) PDB-ID 1QV9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.141 | 0.312 |
Number of reflections | 190905 | |
<I/σ(I)> | 6.5 | 4.4 |
Completeness [%] | 96.7 | 93.6 |
Redundancy | 1.86 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MOPS, KOH, MPD, PEG 400, Mg acetate, Na phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |