1U4L
human RANTES complexed to heparin-derived disaccharide I-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2000-07-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.017, 56.350, 94.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.160 - 2.000 |
Rwork | 0.198 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eqt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX (2002) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.065 | 0.216 |
Number of reflections | 9099 | |
<I/σ(I)> | 20.3 | 6.75 |
Completeness [%] | 98.8 | 98.7 |
Redundancy | 4.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% (w/v) PEG 400, 100mM acetate buffer, 1mM Heparin Disaccharide I-S, 10% (w/v) glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |