1U3E
DNA binding and cleavage by the HNH homing endonuclease I-HmuI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 45.000, 108.300, 248.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.920 |
R-factor | 0.22 |
Rwork | 0.216 |
R-free | 0.24700 |
Structure solution method | SAD |
Starting model (for MR) | pdb from SAD phase and DM with solvent flipping |
RMSD bond length | 0.007 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.960 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.433 | |
Number of reflections | 13360 | |
<I/σ(I)> | 23.3 | 2.4 |
Completeness [%] | 99.4 | 97 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Hanging drop vapor diffusion | 8.75 | 298 | PEG600, pH 8.75, Hanging drop vapor diffusion, temperature 298K |
1 | Hanging drop vapor diffusion | 8.75 | 298 | PEG600, pH 8.75, Hanging drop vapor diffusion, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG600 | ||
2 | 1 | 1 | H2O | ||
3 | 1 | 2 | PEG600 | ||
4 | 1 | 2 | PEG600 |