1U3E
DNA binding and cleavage by the HNH homing endonuclease I-HmuI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 45.000, 108.300, 248.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.920 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| Starting model (for MR) | pdb from SAD phase and DM with solvent flipping |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.960 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.433 | |
| Number of reflections | 13360 | |
| <I/σ(I)> | 23.3 | 2.4 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging drop vapor diffusion | 8.75 | 298 | PEG600, pH 8.75, Hanging drop vapor diffusion, temperature 298K |
| 1 | Hanging drop vapor diffusion | 8.75 | 298 | PEG600, pH 8.75, Hanging drop vapor diffusion, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG600 | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | PEG600 | ||
| 4 | 1 | 2 | PEG600 |
