1U3C
Crystal Structure of the PHR domain of Cryptochrome 1 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 169.430, 169.430, 104.245 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.600 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | E.coli DNA photolyase pdb entry 1DNP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.094 | 0.806 |
| Number of reflections | 26452 | |
| <I/σ(I)> | 26.5 | 2.8 |
| Completeness [%] | 96.5 | 97.6 |
| Redundancy | 12.4 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 309 | glycerol, MES, sulfobetaine-195, 1,6-hexanediol, sodium, potassium tartrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 309K |
