1U12
M. loti cyclic nucleotide binding domain mutant
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.964, 0.979, 0.9788 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.126, 57.126, 193.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.390 - 2.700 |
R-factor | 0.2533 |
Rwork | 0.253 |
R-free | 0.28140 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.390 | 2.870 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 16180 | |
<I/σ(I)> | 6 | |
Completeness [%] | 96.5 | 98.5 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | HEPES buffer, NaCl, Potassium Thiocyanate, Ammonium Sulphate, Ethylene Glycol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |