1U12
M. loti cyclic nucleotide binding domain mutant
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.964, 0.979, 0.9788 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.126, 57.126, 193.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.390 - 2.700 |
| R-factor | 0.2533 |
| Rwork | 0.253 |
| R-free | 0.28140 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.390 | 2.870 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 16180 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 96.5 | 98.5 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | HEPES buffer, NaCl, Potassium Thiocyanate, Ammonium Sulphate, Ethylene Glycol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
