1U04

Crystal structure of full length Argonaute from Pyrococcus furiosus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	69.726, 104.188, 74.015
Unit cell angles	90.00, 102.83, 90.00

Refinement procedure

Resolution	38.170 - 2.250
R-factor	0.227
Rwork	0.227
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.170	2.390
High resolution limit [Å]	2.250	2.250
Number of reflections	48822
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	290	alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 8.00