1U04
Crystal structure of full length Argonaute from Pyrococcus furiosus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.726, 104.188, 74.015 |
Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
Resolution | 38.170 - 2.250 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.170 | 2.390 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 48822 | |
Completeness [%] | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 290 | alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 8.00 | |
1 | 8 | 290 | alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 8.00 | |
1 | 8 | 290 | alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 8.00 |