1TZQ
Crystal structure of the equinatoxin II 8-69 double cysteine mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.585, 52.682, 39.789 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iaz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | MAIN |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG 4000, ammonium sulphate, Na-acetate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
