1TZO
Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0800 |
Spacegroup name | P 1 |
Unit cell lengths | 149.608, 167.028, 168.200 |
Unit cell angles | 77.56, 75.74, 76.01 |
Refinement procedure
Resolution | 30.000 - 3.600 |
R-factor | 0.315 |
Rwork | 0.314 |
R-free | 0.31700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4.5 PA heptamer model |
RMSD bond length | 0.005 |
RMSD bond angle | 1.097 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 163256 | |
<I/σ(I)> | 10.6 | 1.6 |
Completeness [%] | 93.4 | 68.8 |
Redundancy | 2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 298 | HEPES, sodium chloride, b-octylglucoside, spermine, pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |