1TZD
CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE
Experimental procedure
| Experimental method | MAD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.679, 98.535, 188.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.920 - 2.200 |
| R-factor | 0.25992 |
| Rwork | 0.257 |
| R-free | 0.30520 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.766 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 32563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.2M AMMONIUM sulfate, 0.2M SODIUM FORMATE, 10 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
