1TYH
Crystal Structure of Transcriptional Activator tenA from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97959, 0.97991, 0.94 |
| Spacegroup name | P 41 |
| Unit cell lengths | 58.430, 58.430, 297.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.540 |
| R-factor | 0.2437 |
| Rwork | 0.244 |
| R-free | 0.30390 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.066 | 0.401 |
| Number of reflections | 32993 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.6 | 96.6 |
| Redundancy | 14.7 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 298 | PEG 4000, Sodium Citrate, Glycerol, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
