1TY0
Crystal structure of the streptococcal pyrogenic exotoxin J (SPE-J)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.632, 46.348, 72.209 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.750 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Superantigen SPE-C |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.730 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.453 |
| Number of reflections | 72162 | |
| <I/σ(I)> | 7.9 | 1.9 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | lithium acetate, PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
