1TXF
CRYSTAL STRUCTURE OF THE GLUR5 LIGAND BINDING CORE IN COMPLEX WITH GLUTAMATE AT 2.1 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99997 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.809, 63.710, 50.354 |
Unit cell angles | 90.00, 107.22, 90.00 |
Refinement procedure
Resolution | 32.400 - 2.100 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.045 | 0.269 |
Number of reflections | 21345 | |
<I/σ(I)> | 12.7 | 2.2 |
Completeness [%] | 84.3 | 49.7 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 10K, 100 mM HEPES, 20 mM NaCl, 1 mM EDTA, 10 mM Glutamate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |