1TX4
RHO/RHOGAP/GDP(DOT)ALF4 COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLAGE |
| Collection date | 1997-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.500, 72.000, 91.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.650 |
| R-factor | 0.169 * |
| Rwork | 0.169 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1am4 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 1.720 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.065 | 0.324 * |
| Total number of observations | 160124 * | |
| Number of reflections | 42992 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 80.5 | 60.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | PROTEIN WAS CRYSTALLIZED FROM 18% PEG 2000 MME 100MM MES PH6.0 10MM MGCL2, 3MM DTT, 3MM NAN3, 114MM AMMONIUM SULFATE 400UM PROTEIN COMPLEX WITH 1MM ALCL3 AND 10MM NAF. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 18 (%) | |
| 10 | 1 | drop | 10 (mM) | ||
| 2 | 1 | reservoir | MES | 100 (mM) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 5 | 1 | reservoir | 3 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 114 (mM) | |
| 7 | 1 | drop | protein | 0.400 (mM) | |
| 8 | 1 | drop | Tris-HCl | 20 (mM) | |
| 9 | 1 | drop | 1 (mM) |
