1TW8
HincII bound to Ca2+ and cognate DNA GTCGAC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 66.945, 177.180, 254.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.180 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kc6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.140 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.066 |
Number of reflections | 36795 |
<I/σ(I)> | 5.3 |
Completeness [%] | 96.9 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | PEG 4000, 0.1M citrate, 0.15M NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |