1TW8

HincII bound to Ca2+ and cognate DNA GTCGAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	I 21 21 21
Unit cell lengths	66.945, 177.180, 254.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.180
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kc6
RMSD bond length	0.008
RMSD bond angle	1.140
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.066
Number of reflections	36795
<I/σ(I)>	5.3
Completeness [%]	96.9
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	PEG 4000, 0.1M citrate, 0.15M NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K