1TVG
X-ray structure of human PP25 gene product, HSPC034. Northeast Structural Genomics Target HR1958.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97896 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.974, 41.617, 46.779 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 29.740 - 1.600 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24400 |
| Structure solution method | SAD for Sm derivative |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.407 | |
| Number of reflections | 48903 | |
| <I/σ(I)> | 18.47 | 1.65 |
| Completeness [%] | 60 | |
| Redundancy | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG, 200 mM CaCl(2), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
