1TVE
Homoserine Dehydrogenase in complex with 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 95 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0722 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.456, 54.379, 92.497 |
Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
Resolution | 35.240 - 3.000 |
R-factor | 0.289 |
Rwork | 0.289 |
R-free | 0.36300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ebu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.240 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.085 |
Number of reflections | 14617 |
<I/σ(I)> | 7.3 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 295 | Ches, PEG 600, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |