1TVB
Crystal structure of Melanoma Antigen gp100(209-217) Bound to Human Class I MHC HLA-A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.388, 84.452, 84.016 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.16743 |
| Rwork | 0.165 |
| R-free | 0.21618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.946 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 0.344 |
| Number of reflections | 74663 | |
| <I/σ(I)> | 16.8 | 3.7 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 3350, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
