1TV9
HUMAN DNA POLYMERASE BETA COMPLEXED WITH NICKED DNA CONTAINING A MISMATCHED TEMPLATE ADENINE AND INCOMING CYTIDINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.680, 79.650, 54.670 |
| Unit cell angles | 90.00, 106.77, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.400 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.186 | 0.186 |
| Number of reflections | 29484 | |
| <I/σ(I)> | 23.4 | 4.38 |
| Completeness [%] | 75.1 | |
| Redundancy | 3.4 | 2.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 290 | 14% PEG 3350, 350mM sodium Acetate, 50mM imidazole, 10mM MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K, pH 7.50 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium Acetate | ||
| 3 | 1 | 1 | imidazole | ||
| 4 | 1 | 1 | MgCl2 |
