1TTU
Crystal Structure of CSL bound to DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9793, 0.9795, 0.9686 |
| Spacegroup name | P 61 |
| Unit cell lengths | 127.547, 127.547, 97.604 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 2.850 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.046 | 0.313 |
| Number of reflections | 22264 | |
| <I/σ(I)> | 23.2 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | MES, ammonium sulfate, PEG 20K, ethylene glycol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | MES, ammonium sulfate, PEG 20K, ethylene glycol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | PEG 20K | ||
| 4 | 1 | 1 | ethylene glycol |
