1TTT

Phe-tRNA, elongation factoR EF-TU:GDPNP ternary complex

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-03-10
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	206.835, 122.354, 151.552
Unit cell angles	90.00, 126.30, 90.00

Refinement procedure

Resolution	25.000 - 2.700
R-factor	0.206 *
Rwork	0.206
R-free	0.29600 *
RMSD bond length	0.010
RMSD bond angle	17.300 *
Data reduction software	DENZO
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.700
Rmerge	0.052
Number of reflections	80769
Completeness [%]	96.8 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.8	4 *	Nissen, P., (1994) FEBS Lett., 356, 165. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	TC	15 (mg/ml)
2	1	reservoir	AS	47-49 (%)