1TT9
Structure of the bifunctional and Golgi associated formiminotransferase cyclodeaminase octamer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2003-11-04 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9791, 0.9793, 0.9716 |
| Spacegroup name | P 4 |
| Unit cell lengths | 134.848, 134.848, 156.365 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.420 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29300 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.060 | 0.249 |
| Number of reflections | 31393 | |
| <I/σ(I)> | 6 | 1.9 |
| Completeness [%] | 86.6 | 64.7 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 600, sodium sulphate, HEPES, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
