1TSQ

CRYSTAL STRUCTURE OF AP2V SUBSTRATE VARIANT OF NC-P1 DECAMER PEPTIDE IN COMPLEX WITH V82A/D25N HIV-1 PROTEASE MUTANT

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	220
Detector technology	IMAGE PLATE
Collection date	2002-07-12
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
格子定数 [Å]	50.771, 57.258, 60.917
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	37.990 - 2.000
R因子	0.195
Rwork	0.195
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mtr
結合長の平均二乗偏差(RMSD) [Å]	0.017
結合角の平均二乗偏差(RMSD) [度]	1.300
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5)

Quality characteristics

	Overall
分解能 [Å] (低)	37.990
分解能 [Å] (高)	2.000
Rmerge_l_obs	0.075
独立反射数	12492
完全性 [%]	99.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	SODIUM PHOSPHATE, SODIUM CITRATE, AMMONIUM SULPHATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K