1TQC
Ovine recombinant PrP(114-234), ARR variant in complex with the VRQ14 Fab fragment (IgG2a)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Detector technology | CCD |
Collection date | 2003-08-22 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.659, 145.448, 43.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.950 - 2.800 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.087 | 0.375 |
Number of reflections | 15206 | |
<I/σ(I)> | 17.1 | 4.2 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | Sodium citrate 0.1M , PEG8000, Ammonium acetate 0.2M, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |