1TPX
Ovine recombinant PrP(114-234), ARQ variant in complex with the Fab of the VRQ14 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Detector technology | CCD |
| Collection date | 2002-12-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979774 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.770, 145.593, 43.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.560 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.030 | 0.100 |
| Number of reflections | 18975 | |
| <I/σ(I)> | 55.1 | 12 |
| Completeness [%] | 98.3 | 94.6 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | Sodium citrate 0.1M, PEG8000, Ammonium acetate 0.2M, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
