1TP7
Crystal Structure of the RNA-dependent RNA Polymerase from Human Rhinovirus 16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.428, 118.890, 183.107 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.053 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.463 |
| Number of reflections | 98759 | |
| <I/σ(I)> | 12.9 | 3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | PEG 4000, ammonium sulfate, dimethylbenzylammonium propane sulfonate, sodium citrate, glycerol, dithiothreitol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
