1TP7
Crystal Structure of the RNA-dependent RNA Polymerase from Human Rhinovirus 16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Wavelength(s) | 0.97887 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.428, 118.890, 183.107 |
Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.29200 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.053 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.463 |
Number of reflections | 98759 | |
<I/σ(I)> | 12.9 | 3 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | PEG 4000, ammonium sulfate, dimethylbenzylammonium propane sulfonate, sodium citrate, glycerol, dithiothreitol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |