1TP0
Triple mutation in interleukin 1 beta cavity:replacement of phenylalanines with tryptophan.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 54.970, 54.970, 76.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.900 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 76.900 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 10627 |
| Completeness [%] | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 29% AMMONIUM SULFATE W/V, 100 MM TRIS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
