1TOM

ALPHA-THROMBIN COMPLEXED WITH HIRUGEN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	71.250, 72.190, 73.110
Unit cell angles	90.00, 101.85, 90.00

Refinement procedure

Resolution	6.000 * - 1.800
R-factor	0.172
Rwork	0.172
RMSD bond length	0.014
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.079 *
Total number of observations	40601 *
Number of reflections	23015 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	thrombin-hirugen complex	3.7 (mg/ml)
2	1	drop	sodium phosphate	0.05 (M)
3	1	drop		0.375 (M)
4	1	drop		0.5 (mM)
5	1	reservoir	phosphate	0.1 (M)
6	1	reservoir		1 (mM)
7	1	reservoir	PEG8000	30 (%(w/v))