1TOM
ALPHA-THROMBIN COMPLEXED WITH HIRUGEN
Experimental procedure
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.250, 72.190, 73.110 |
Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
Resolution | 6.000 * - 1.800 |
R-factor | 0.172 |
Rwork | 0.172 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.900 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.079 * |
Total number of observations | 40601 * |
Number of reflections | 23015 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.3 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | thrombin-hirugen complex | 3.7 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 0.05 (M) | |
3 | 1 | drop | 0.375 (M) | ||
4 | 1 | drop | 0.5 (mM) | ||
5 | 1 | reservoir | phosphate | 0.1 (M) | |
6 | 1 | reservoir | 1 (mM) | ||
7 | 1 | reservoir | PEG8000 | 30 (%(w/v)) |