1TNY
Rat Protein Geranylgeranyltransferase Type-I Complexed with a GGPP analog and a FREKKFFCAIL Peptide Derived from the Heterotrimeric G Protein Gamma-2 Subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.006 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 270.947, 264.007, 184.985 |
Unit cell angles | 90.00, 131.72, 90.00 |
Refinement procedure
Resolution | 29.910 - 2.700 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n4q |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 250142 | |
<I/σ(I)> | 15 | 2.5 |
Completeness [%] | 94.1 | 82.6 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | 1.3 M ammonium sulfate, 175 mM NaCitrate pH 6.5, 100 mM MES pH 6.3, 20 mM DTT, 1uM ZnCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |