1TNB

Rat Protein Geranylgeranyltransferase Type-I Complexed with a GGPP analog and a substrate KKSKTKCVIF Peptide Derived from TC21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9000
Spacegroup name	C 1 2 1
Unit cell lengths	271.130, 266.720, 185.141
Unit cell angles	90.00, 131.68, 90.00

Refinement procedure

Resolution	27.660 - 2.850
R-factor	0.189
Rwork	0.189
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n4q
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	217761
<I/σ(I)>	11.1	2.7
Completeness [%]	95.6	88.8
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	1.3 M ammonium sulfate, 175 mM NaCitrate pH 6.5, 100 mM MES pH 6.3, 20 mM DTT, 1uM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K