Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1TKD

T7 DNA polymerase ternary complex with 8 oxo guanosine and dCMP at the elongation site

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X26C
Synchrotron siteNSLS
BeamlineX26C
Temperature [K]100
Detector technologyCCD
Collection date2004-01-28
DetectorADSC QUANTUM 4
Wavelength(s)1
Spacegroup nameP 21 21 2
Unit cell lengths105.514, 215.112, 52.035
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.910 - 2.490
R-factor0.202
Rwork0.202
R-free0.26500
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1t7p
RMSD bond length0.005
RMSD bond angle1.200
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.570
High resolution limit [Å]2.4902.480
Number of reflections40910
<I/σ(I)>24.68.4
Completeness [%]96.486
Redundancy5.94.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5294PEG8000, Ammonium Sulfate, PEG400, Ethylene Glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

225158

PDB entries from 2024-09-18

PDB statisticsPDBj update infoContact PDBjnumon