1TKD
T7 DNA polymerase ternary complex with 8 oxo guanosine and dCMP at the elongation site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.514, 215.112, 52.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.910 - 2.490 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t7p |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.490 | 2.480 |
Number of reflections | 40910 | |
<I/σ(I)> | 24.6 | 8.4 |
Completeness [%] | 96.4 | 86 |
Redundancy | 5.9 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | PEG8000, Ammonium Sulfate, PEG400, Ethylene Glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |